The Effects of Nitric Oxide Synthase Inhibitors in Acetaminophen-Induced Hepatic Injury in Mice

(1) Aim of the Study: In this study, we aimed to evaluate vascular damage and effects nitric oxide synthase (NOS) enzyme inhibitors in hepatic caused by high doses acetaminophen (APAP). (2) Material methods: Fifty-three Swiss albino male mice were used for study. Hepatic thoracic aorta toxicity 2 or 6 h exposures APAP (300 mg/kg intraperitoneally (i.p.)) evaluated. The general NOS inhibitor NG-nitro-L-arginine methyl ester (L-NAME: 25 50 mg/kg, i.p.) neuronal 7-nitroindazole (7-NI: 15 administered one hour before exposure. (3) Results: Significant morphological deteriorations observed after exposure histopathological examinations sections. Pre-treatment with L-NAME (at mg/kg) 7-NI a significantly decreased (p < 0.05). increases ALT levels both (with but not at pre-treatments. No significant change was measured nitrate/nitrite total antioxidant status either serum liver homogenates. during hematoxylin-eosin immunohistochemical staining artery sections, no statistical difference found acetylcholine-mediated relaxation, which may indicate endothelial dysfunction. (4) Conclusions: This study demonstrated that APAP-induced toxicity, especially inhibitors, decrease toxicity. It also shown accompanied